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Microarray studies using Affymetrix gene chips have demonstrated GHK-Cu resetting aging-related gene expression profiles in dermal fibroblasts, including upregulation of genes encoding superoxide dismutase (SOD1), metallothionein, collagen types I and III, and multiple anti-inflammatory interleukins
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PV2 and PV3 nanobody mutants with pAcF incorporated were purified and verified by SDS-PAGE and mass spectrometry to ensure correct insertion of the ncAA (Figures S3&4)
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